General description

An internally quenched, highly cathepsin S-selective, fluorogenic peptide substrate that is efficiently cleaved by CatS (k_cat/K_m = 1.018 µM^-1 S^-1), but not by Cathepsin B & L (k_cat/K_m = 0.004 and 0.032 µM^-1 S^-1, respectively). Studies using CatS-specific inhibitor LHVS reveal that the substrate is not cleaved by other proteolytic enzymes, including CatB, CatH, CatL, and CatX, present in endosomal extracts from human B cell line WT100, dendritic cells, and macrophages (excitation maximum: 340 nm; emission maximum: 405 nm).

An internally quenched, highly cathepsin S-selective, fluorogenic peptide substrate that is efficiently cleaved by CatS (k_cat/K_m = 1.018 µM^-1 S^-1), but not by Cathepsin B & L (k_cat/K_m = 0.004 and 0.032 µM^-1 S^-1, respectively). Studies using CatS-specific inhibitor LHVS reveal that the substrate is not cleaved by other proteolytic enzymes, including CatB, CatH, CatL, and CatX, present in endosomal extracts from human B cell line WT100, dendritic cells, and macrophages. (excitation maximum: 340 nm; emission maximum: 405 nm)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lutzner, N., and Kalbacher, H. 2008. J. Biol. Chem.283, 36185.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Sequence

Mca-Gly-Arg-Trp-Pro-Pro-Met-Gly~Leu-Pro-Trp-Glu-Lys(Dnp)-D-Arg-NH₂

Warning

Toxicity: Standard Handling (A)